Models for recovering the energy landscape of conformational transitions from single-molecule pulling experiments
نویسنده
چکیده
Single-molecule force spectroscopy is a powerful experimental technique for probing intermolecular forces and conformational transitions of individual molecules. This technique involves measuring the mechanical response of a molecule subjected to a constant or time-varying force. Statistical mechanics has played a pivotal role in interpreting forcemeasurements in terms of the underlying kinetics and energy landscape of themolecular transition being studied. Here, we provide a didactic review of various statistical–mechanical models used for analysing these measurements, emphasising the theoretical ideas and assumptions used in deriving these models. ARTICLE HISTORY Received 14 September 2015 Accepted 14 November 2015
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